Portfolio

Upcoming Event

Johnson & Johnson Mechanical Testing and Analysis Summit 
Robb Colbrunn, Ph.D., Guest Speaker
Director of BioRobotics and Mechanical Testing Core
Medical Device Solutions (MDS) Cleveland Clinic
Adjunct Assistant Professor, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University
September 18-20, 2018
Syracuse, IN

BME GUEST SPEAKER SEMINAR
"Back to Metabolism Basics:Transport and Bioenergetics in Tumors and Brain Endothelial Cells"
Lester R. Drewes, Ph.D.
Professor of 
Biochemistry & Molecular Biology
Director, Graduate Programs, MSD
Department of Biomedical Sciences
University of Minnesota Medical School Duluth
Hosted by Chaitali Ghosh, PhD.
September 28, 2018



Surface Contaminants Inhibit Osseointegration in a Novel Murine Model

Client: Edward Greenfeld Ph.D. / Case Western Reserve University

Services Provided: Experiment Design / Uniaxial Material Testing / Data Analysis / Manuscript Preparation

Surface contaminants, such as bacterial debris and manufacturing residues, may remain on orthopaedic implants after sterilization procedures and affect osseointegration. The goals of this study were to develop a murine model of osseointegration in order to determine whether removing surface contaminants enhances osseointegration. To develop the murine model, titanium alloy implants were implanted into a unicortical pilot hole in the mid-diaphysis of the femur and osseointegration was measured over a five week time course. Histology, backscatter scanning electron microscopy and x-ray energy dispersive spectroscopy showed areas of bone in intimate physical contact with the implant, confirming osseointegration. Histomorphometric quantification of bone-to-implant contact and peri-implant bone and biomechanical pullout quantification of ultimate force, stiffness and work to failure increased significantly over time, also demonstrating successful osseointegration. We also found that a rigorous cleaning procedure significantly enhances bone-to-implant contact and biomechanical pullout measures by two-fold compared with implants that were autoclaved, as recommended by the manufacturer. The most likely interpretation of these results is that surface contaminants inhibit osseointegration. The results of this study justify the need for the development of better detection and removal techniques for contaminants on orthopaedic implants and other medical devices.

Publications: http://www.ncbi.nlm.nih.gov/pubmed/21801863